abstract：:The genetic determinants underlying susceptibility to acute lung injury have not been identified. Recently, we found that the strain distribution pattern for mean survival time (MST) to three irritants-ozone, ultrafine Teflon, and nickel sulfate- was shared between inbred mouse strains. For ozone-induced acute lung in...

journal_title：American journal of respiratory cell and molecular biology

authors： Prows DR,Leikauf GD

更新日期：2001-06-01 00:00:00

abstract：:In this study we examined the effect of oral antigen (Ag) administration on the development of experimental asthma in different mouse strains. We selected BALB/c, BP2, CBA/Ca interleukin (IL)-5 transgenic, and BALB/c T-cell receptor-delta-deficient mouse strains because they exhibit different aspects of the asthma syn...

journal_title：American journal of respiratory cell and molecular biology

authors： Russo M,Nahori MA,Lefort J,Gomes E,de Castro Keller A,Rodriguez D,Ribeiro OG,Adriouch S,Gallois V,de Faria AM,Vargaftig BB

更新日期：2001-05-01 00:00:00

abstract：:Tissue inhibitors of metalloproteinases (TIMPs) are multifunctional proteins that have the capacity to modify cellular activities and to modulate matrix turnover. We demonstrate that TIMP-1 messenger RNA (mRNA) and protein expression are selectively and markedly increased in a murine model of bleomycin-induced pulmona...

journal_title：American journal of respiratory cell and molecular biology

authors： Madtes DK,Elston AL,Kaback LA,Clark JG

更新日期：2001-05-01 00:00:00

abstract：:The chemokine TARC is a ligand for the chemokine receptor CCR4 expressed on T helper (Th)2-type CD4 T cells. Allergic airway inflammation is characterized by a local increase in cells secreting Th2-type cytokines. We hypothesized that bronchial epithelial cells may be a source of chemokines known to chemoattract Th2 c...

journal_title：American journal of respiratory cell and molecular biology

authors： Berin MC,Eckmann L,Broide DH,Kagnoff MF

更新日期：2001-04-01 00:00:00

abstract：:To examine the effects of acid exposure on the adherence of Streptococcus pneumoniae to cultured human tracheal epithelial cells, cells were exposed to acid at various pH levels, and various concentrations of S. pneumoniae were added to the culture medium. The number of S. pneumoniae adhering to cultured human trachea...

journal_title：American journal of respiratory cell and molecular biology

authors： Ishizuka S,Yamaya M,Suzuki T,Nakayama K,Kamanaka M,Ida S,Sekizawa K,Sasaki H

更新日期：2001-04-01 00:00:00

abstract：:In these studies, we examined signaling through the transcription factor STAT5 in human peripheral blood eosinophils after treatment with granulocyte macrophage colony-stimulating factor (GM-CSF) or interleukin (IL)-5. In response to either cytokine, STAT5 was rapidly tyrosine phosphorylated and acquired interferon ga...

journal_title：American journal of respiratory cell and molecular biology

authors： Bhattacharya S,Stout BA,Bates ME,Bertics PJ,Malter JS

更新日期：2001-03-01 00:00:00

abstract：:Endotoxin (lipopolysaccharide [LPS]) is known to induce the production of tumor necrosis factor (TNF)-alpha and the induction of manganese superoxide dismutase (MnSOD). We have recently demonstrated that induction of TNF-alpha and MnSOD by LPS is mediated through different signal transduction pathways. In the current ...

journal_title：American journal of respiratory cell and molecular biology

authors： White JE,Tsan MF

更新日期：2001-02-01 00:00:00

abstract：:Two isoforms of the human glucocorticoid receptor (hGR) have been described, hGRalpha and hGRbeta. We analyzed the expression and regulation of both hGR isoforms in human respiratory epithelial cells (BEAS-2B, A549, and primary nasal epithelial cells). In BEAS-2B cells, the expression of hGRalpha messenger RNA (mRNA) ...

journal_title：American journal of respiratory cell and molecular biology

authors： Pujols L,Mullol J,Pérez M,Roca-Ferrer J,Juan M,Xaubet A,Cidlowski JA,Picado C

更新日期：2001-01-01 00:00:00

abstract：:Although several studies have demonstrated that the pulmonary collectins surfactant protein (SP)-A and SP-D contribute to innate immunity by enhancing pathogen phagocytosis, the role of SP-A and SP-D in regulating production of free radicals and cytokines is controversial. We hypothesized that the state and mechanism ...

journal_title：American journal of respiratory cell and molecular biology

authors： Stamme C,Walsh E,Wright JR

更新日期：2000-12-01 00:00:00

abstract：:Endothelin-1 is a potent bronchoconstrictor peptide with pro-inflammatory and growth-promoting properties. After exposure of sensitized Brown-Norway rats to six repeated ovalbumin exposures, there was an increase in pro-endothelin (ET)-1 messenger RNA compared with saline-exposed control rats 24 h after the final expo...

journal_title：American journal of respiratory cell and molecular biology

authors： Salmon M,Liu YC,Mak JC,Rousell J,Huang TJ,Hisada T,Nicklin PL,Chung KF

更新日期：2000-11-01 00:00:00

abstract：:Bleomycin-induced lung injury causes increased fibroblast numbers in the lung and pulmonary fibrosis. Studies of fibroblasts isolated from such injured lungs have revealed evidence of increased intrinsic proliferative capacity, but the mechanism is unknown. Telomerase catalyzes the addition of telomeric DNA repeats on...

journal_title：American journal of respiratory cell and molecular biology

authors： Nozaki Y,Liu T,Hatano K,Gharaee-Kermani M,Phan SH

更新日期：2000-10-01 00:00:00

abstract：:Identification of the genetic loci underlying asthma in humans has been hampered by variability in clinical phenotype, uncontrolled environmental influences, and genetic heterogeneity. To circumvent these complications, the genetic regulation of asthma-associated phenotypes was studied in a murine model. We characteri...

journal_title：American journal of respiratory cell and molecular biology

authors： Ewart SL,Kuperman D,Schadt E,Tankersley C,Grupe A,Shubitowski DM,Peltz G,Wills-Karp M

更新日期：2000-10-01 00:00:00

abstract：:Airway epithelial cells can respond to infection by activating several signaling pathways. We examined the induction of apoptosis in response to Pseudomonas aeruginosa PAO1 in normal cells and several cystic fibrosis (CF) and corrected cell lines. Epithelial cells in monolayers with tight junctions, confirmed by apica...

journal_title：American journal of respiratory cell and molecular biology

authors： Rajan S,Cacalano G,Bryan R,Ratner AJ,Sontich CU,van Heerckeren A,Davis P,Prince A

更新日期：2000-09-01 00:00:00

abstract：:In bronchial asthma, eosinophils found in the airways have an enhanced inflammatory capacity. We hypothesized that, at least in part, changes in functional phenotype are due to the effect of transendothelial migration. To model in vivo eosinophil trafficking to the lung, we cultured human pulmonary microvascular endot...

journal_title：American journal of respiratory cell and molecular biology

authors： Yamamoto H,Sedgwick JB,Vrtis RF,Busse WW

更新日期：2000-09-01 00:00:00

abstract：:Although neutrophil migration from the systemic circulation involves the beta2- (or CD18) integrin family, the existence of an alternative, CD18-independent route of neutrophil extravasation to tissues has been demonstrated in animal models. The molecular interactions involved in this alternative migratory route have ...

journal_title：American journal of respiratory cell and molecular biology

authors： Mackarel AJ,Russell KJ,Brady CS,FitzGerald MX,O'Connor CM

更新日期：2000-08-01 00:00:00

abstract：:The effects of repeated ozone exposures on the development of immune responses toward ovalbumin (OVA) were investigated in BALB/c and C57BL/6 mice. Ozone exposures (180 to 500 microg/m(3); 4 h, three times/wk for 4 wk) were combined with a protocol of OVA-aerosol exposure (1% OVA). Immediate cutaneous hypersensitivity...

journal_title：American journal of respiratory cell and molecular biology

authors： Neuhaus-Steinmetz U,Uffhausen F,Herz U,Renz H

更新日期：2000-08-01 00:00:00

abstract：:Clara-cell populations show a high degree of variation in susceptibility to injury by bioactivated cytotoxicants. Because glutathione (GSH) is critical for detoxification of electrophilic metabolites, heterogeneity in Clara cell GSH levels may lead to a wide range of cytotoxic responses. This study was designed to def...

journal_title：American journal of respiratory cell and molecular biology

authors： West JA,Chichester CH,Buckpitt AR,Tyler NK,Brennan P,Helton C,Plopper CG

更新日期：2000-07-01 00:00:00

abstract：:The human lung accumulates iron with senescence. Smoking escalates the accumulation of iron, and we have demonstrated regional variability in the accumulation of iron in smokers' lungs. Iron has been reported to influence the production of a number of proinflammatory mediators, including human interleukin (IL)-1beta. ...

journal_title：American journal of respiratory cell and molecular biology

authors： O'Brien-Ladner AR,Nelson SR,Murphy WJ,Blumer BM,Wesselius LJ

更新日期：2000-07-01 00:00:00

abstract：:Interleukin (IL)-9 has recently been shown to play an important role in allergic disease because its expression is strongly associated with the degree of airway responsiveness and the asthmatic-like phenotype. IL-9 is a pleiotropic cytokine that is active on many cell types involved in the allergic immune response. Mu...

journal_title：American journal of respiratory cell and molecular biology

authors： Louahed J,Toda M,Jen J,Hamid Q,Renauld JC,Levitt RC,Nicolaides NC

更新日期：2000-06-01 00:00:00

abstract：:Pneumocystis carinii causes severe pneumonia in immunocompromised patients. Recent studies indicate that P. carinii uses a Cdc2 cyclin-dependent kinase to control its proliferation. To further study the regulation of the life cycle of P. carinii, we characterized the P. carinii B-type cyclin termed Cdc13, whose bindin...

journal_title：American journal of respiratory cell and molecular biology

authors： Kottom TJ,Thomas CF Jr,Mubarak KK,Leof EB,Limper AH

更新日期：2000-06-01 00:00:00

abstract：:Interleukin (IL)-1beta stimulates the release of granulocyte macrophage colony-stimulating factor (GM-CSF) from lung epithelial cells. To investigate the molecular mechanisms underlying GM-CSF regulation, we studied GM-CSF production, messenger RNA (mRNA) expression levels, and GM-CSF promoter activity in A549 human a...

journal_title：American journal of respiratory cell and molecular biology

authors： Bergmann M,Barnes PJ,Newton R

更新日期：2000-05-01 00:00:00

abstract：:We recently described overproduction of interferon (IFN)-gamma by lung lymphocytes in mice with silicosis (11% of cells in air-control versus 19% of cells from silica-exposed mice; Davis and colleagues, Am. J. Respir. Cell Mol. Biol. 1999;20:813-824). We hypothesized that the increased IFN-gamma production might be du...

journal_title：American journal of respiratory cell and molecular biology

authors： Davis GS,Pfeiffer LM,Hemenway DR

更新日期：2000-04-01 00:00:00

abstract：:Airway inflammation, hyperreactivity, increased number of goblet cells, and mucus overproduction characterize asthma. Respiratory challenge with ovalbumin (OVA) of sensitized mice has been shown by several laboratories to cause pulmonary pathology similar to that observed in human allergic asthma. Recently, interleuki...

journal_title：American journal of respiratory cell and molecular biology

authors： Zuhdi Alimam M,Piazza FM,Selby DM,Letwin N,Huang L,Rose MC

更新日期：2000-03-01 00:00:00

abstract：:Mechanisms of protective immunity to mycobacterial infection in the lung remain poorly defined. In this study, T-cell subset expansion and cytokine expression in bronchoalveolar spaces, lung parenchyma, and mediastinal lymph nodes of mice infected intratracheally with Mycobacterium bovis-Calmette-Guerin bacillus (BCG)...

journal_title：American journal of respiratory cell and molecular biology

authors： Fulton SA,Martin TD,Redline RW,Henry Boom W

更新日期：2000-03-01 00:00:00

abstract：:Acute lung injury induced by reactive oxygen gases such as ozone (O(3)) is focal and site-selective. To define patterns of acute epithelial injury along intrapulmonary airways, we developed a new analytic approach incorporating labeling of permeable cells, airway microdissection, and laser scanning confocal microscopy...

journal_title：American journal of respiratory cell and molecular biology

authors： Postlethwait EM,Joad JP,Hyde DM,Schelegle ES,Bric JM,Weir AJ,Putney LF,Wong VJ,Velsor LW,Plopper CG

更新日期：2000-02-01 00:00:00

abstract：:A small proline-rich protein, SPR1, is overexpressed in squamous metaplasia of bronchial epithelium. We studied the expression and regulation of SPR1 in a series of human bronchial epithelial cell lines representing a model of multistep bronchial carcinogenesis. These cell lines included a primary culture of tracheobr...

journal_title：American journal of respiratory cell and molecular biology

authors： Lau D,Xue L,Hu R,Liaw T,Wu R,Reddy S

更新日期：2000-01-01 00:00:00

abstract：:Interleukin (IL)-4 and (IL)-13 induce immunoglobulin (Ig)E synthesis via activation of the transcription factor signal transducer and activator of transcription (Stat)6. The present study describes the identification and characterization of antisense oligonucleotides to Stat6 as an approach to interrupt IL-4 and IL-13...

journal_title：American journal of respiratory cell and molecular biology

authors： Hill S,Herlaar E,Le Cardinal A,van Heeke G,Nicklin P

更新日期：1999-12-01 00:00:00

abstract：:Fetal airway smooth muscle contracts to neural stimulation from early gestation. This study aimed to document the development of the nerves and ganglia within the bronchial tree of the fetal pig lung as the structural correlates for this function. The formation of these structures during lung development (pseudoglandu...

journal_title：American journal of respiratory cell and molecular biology

authors： Weichselbaum M,Sparrow MP

更新日期：1999-11-01 00:00:00

abstract：:Previous studies have shown that the pan CD28/cytotoxic T lymphocyte antigen (CTL)A-4 antagonist CTLA4 immunoglobulin (Ig) inhibits eosinophilic airway inflammation in Schistosoma mansoni-sensitized and airway-challenged mice. In the present study, the importance of CD28 as well as the individual roles of CD80 and CD8...

journal_title：American journal of respiratory cell and molecular biology

authors： Mathur M,Herrmann K,Qin Y,Gulmen F,Li X,Krimins R,Weinstock J,Elliott D,Bluestone JA,Padrid P

更新日期：1999-10-01 00:00:00

abstract：:Pulmonary lymphangioleiomyomatosis (LAM), a disease of young women, is characterized by proliferation of immature-appearing smooth-muscle cells (LAM cells) in the lungs and abdomen. LAM cells react with monoclonal antibody HMB45, which recognizes a 100-kD glycoprotein (gp100) originally found in human melanoma cells. ...

journal_title：American journal of respiratory cell and molecular biology

authors： Matsumoto Y,Horiba K,Usuki J,Chu SC,Ferrans VJ,Moss J

更新日期：1999-09-01 00:00:00

abstract：:Polymorphonuclear leukocytes (PMN) and eosinophils (Eos) are important cellular participants in a variety of acute and chronic inflammatory reactions in the airway. Histologic evidence has implicated direct interactions between these two subsets of leukocytes and airway epithelial cells during inflammation. A comprehe...

journal_title：American journal of respiratory cell and molecular biology

authors： Jagels MA,Daffern PJ,Zuraw BL,Hugli TE

更新日期：1999-09-01 00:00:00

abstract：:Lung elastin synthesis is normally confined to periods of development, is maximal during alveolarization, and declines to low levels in mature lung. We have previously described an elastogenic response in the adult rat lung associated with experimental granulomatous disease induced by silica instillation. Reinitiated ...

journal_title：American journal of respiratory cell and molecular biology

authors： Mariani TJ,Arikan MC,Pierce RA

更新日期：1999-08-01 00:00:00

abstract：:We examined the potential of several epithelial-derived factors to enhance neutrophil activation and survival. Neutrophils incubated in the presence of supernatants from nasal-derived primary epithelial cultures had significantly increased survival compared with neutrophils cultured in media alone. Of the cytokines re...

journal_title：American journal of respiratory cell and molecular biology

authors： Daffern PJ,Jagels MA,Hugli TE

更新日期：1999-08-01 00:00:00

abstract：:The effects of concomitant P1-receptor stimulation on peak intracellular Ca2+ release by extracellular adenosine 5'-triphosphate (ATP) and 5-hydroxytryptamine (5-HT) were investigated in cultured airway smooth-muscle (ASM) cells. The results show that peak Ca2+ release to ATP is enhanced by preincubation with adenosin...

journal_title：American journal of respiratory cell and molecular biology

authors： Michoud MC,Tao FC,Pradhan AA,Martin JG

更新日期：1999-07-01 00:00:00

abstract：:We investigated the effects of hypoxia (< 2.5% O2) on rat manganese superoxide dismutase (MnSOD) gene promoter-luciferase reporter constructs in transiently transfected lung epithelial cells (A549, L2, and E1A-T2) and fibroblasts (R9Ab). We cloned MnSOD promoter-luciferase reporter constructs (numbers refer to length ...

journal_title：American journal of respiratory cell and molecular biology

authors： Ohman T,Parish G,Jackson RM

更新日期：1999-07-01 00:00:00

abstract：:Idiopathic pneumonia syndrome (IPS) is a significant clinical problem encountered among patients treated with bone marrow transplantation (BMT). IPS is identified as an inflammatory lung disease characterized by diffuse interstitial pneumonitis and alveolitis leading to interstitial fibrosis in the absence of an ident...

journal_title：American journal of respiratory cell and molecular biology

authors： Shankar G,Scott Bryson J,Darrell Jennings C,Kaplan AM,Cohen DA

更新日期：1999-06-01 00:00:00

abstract：:It has long been speculated that neutrophils deploy proteases to digest subendothelial matrix as they migrate from the bloodstream. Direct evidence for the involvement of proteases in neutrophil transendothelial migration is, however, lacking. To address this issue we used transmission electron microscopy to verify th...

journal_title：American journal of respiratory cell and molecular biology

authors： Mackarel AJ,Cottell DC,Russell KJ,FitzGerald MX,O'Connor CM

更新日期：1999-06-01 00:00:00

abstract：:Polymorphonuclear leukocytes (PMN) release gelatinase B in response to variable stimuli. Gelatinase B degrades basement membrane components in vitro, and inhibition of matrix metalloproteinase activity blunts PMN migration through a prototype basement membrane (Matrigel) and amnionic membranes. Accordingly, it has bee...

journal_title：American journal of respiratory cell and molecular biology

authors： Betsuyaku T,Shipley JM,Liu Z,Senior RM

更新日期：1999-06-01 00:00:00

abstract：:Alveolar macrophages (AM) express gelatinase B, a member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components. We evaluated the expression of gelatinase B in the course of idiopathic pulmonary fibrosis (IPF) by studying alveolar macrophages in culture AM ...

journal_title：American journal of respiratory cell and molecular biology

authors： Lemjabbar H,Gosset P,Lechapt-Zalcman E,Franco-Montoya ML,Wallaert B,Harf A,Lafuma C

更新日期：1999-05-01 00:00:00

abstract：:Human papillomaviruses (HPVs) such as types 6 and 11 can establish lifelong infections in airway epithelial cells in patients, and long-term infection can lead to pulmonary involvement and death. The mechanisms underlying this persistence depend on both the transcriptional activity of the viral enhancers and promoters...

journal_title：American journal of respiratory cell and molecular biology

authors： Gadi VK,Zou N,Liu JS,Cheng S,Broker TR,Sorscher EJ,Chow LT

更新日期：1999-05-01 00:00:00